› 7e14

Electron Microscopy
2.9Å resolution

Compound2_GLP-1R_OWL833_Gs complex structure

Released:
DOI: 10.2210/pdb7e14/pdb
PDB entry 7e14 coloured by chain, front view.
PDB entry 7e14 coloured by chain, side view.
PDB entry 7e14 coloured by chain, top view.
Source organisms:
Primary publication:
Molecular insights into ago-allosteric modulation of the human glucagon-like peptide-1 receptor.
Cong Z, Chen LN, Ma H, Zhou Q, Zou X, Ye C, Dai A, Liu Q, Huang W, Sun X, Wang X, Xu P, Zhao L, Xia T, Zhong W, Yang D, Eric Xu H, Zhang Y, Wang MW
Nat Commun 12 3763 (2021)
PMID: 34145245
Related structures: EMD-30936

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-211236 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 28.99 KDa
Source organism: Homo sapiens
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 62%)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 345 amino acids
Theoretical weight: 37.92 KDa
Source organism: Bos taurus
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Rattus norvegicus
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nb35 Chain: N
Molecule details ›
Chain: N
Length: 128 amino acids
Theoretical weight: 13.89 KDa
Source organism: synthetic construct
Expression system: Insect cell expression vector pTIE1
Glucagon-like peptide 1 receptor Chain: R
Molecule details ›
Chain: R
Length: 440 amino acids
Theoretical weight: 50.86 KDa
Source organism: Homo sapiens
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: P43220 (Residues: 24-463; Coverage: 100%)
Gene name: GLP1R
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand V6G 1 x V6G
Ligand CLR 5 x CLR
Ligand HNO 1 x HNO
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-30936
Expression system: Insect cell expression vector pTIE1

Quick links

Citations

3 review citations

Structure, function and drug discovery of GPCR signaling.
Cheng et al. (2023)
2 more

5 mentions without citation

G protein-coupled receptors in neurodegenerative diseases and psychiatric disorders.
Wong et al. (2023)
4 more