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› 7e1w

Electron Microscopy
2.67Å resolution

Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203

Released:
DOI: 10.2210/pdb7e1w/pdb
PDB entry 7e1w coloured by chain, front view.
PDB entry 7e1w coloured by chain, side view.
PDB entry 7e1w coloured by chain, top view.
Source organisms:
Primary publication:
Structure of Mycobacterium tuberculosis cytochrome bcc in complex with Q203 and TB47, two anti-TB drug candidates.
Zhou S, Wang W, Zhou X, Zhang Y, Lai Y, Tang Y, Xu J, Li D, Lin J, Yang X, Ran T, Chen H, Guddat LW, Wang Q, Gao Y, Rao Z, Gong H
OpenAccess logo Elife 10 (2021)
PMID: 34819223
Related structures: EMD-30944

Function and Biology Details

Reactions catalysed: 
4 Fe(II)-[cytochrome c] + O2 + 8 H(+)(in) = 4 Fe(III)-[cytochrome c] +2 H2O + 4 H(+)(out).
a quinol + 2 Fe(III)-[cytochrome c](out) = a quinone + 2 Fe(II)-[cytochrome c](out) + 2 H(+)(out).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero icosamer (preferred)
PDBe Complex ID:
PDB-CPX-279089 (preferred)
Entry contents:
10 distinct polypeptide molecules
Macromolecules (10 distinct):
cytochrome-c oxidase Chains: E, Q
Molecule details ›
Chains: E, Q
Length: 341 amino acids
Theoretical weight: 38.08 KDa
Source organism: Mycolicibacterium smegmatis MC2 51
UniProt:
  • Canonical: A0R057 (Residues: 1-341; Coverage: 100%)
Gene name: MSMEG_4268
Sequence domains: Cytochrome C oxidase subunit II, periplasmic domain
Cytochrome c oxidase subunit 1 Chains: F, R
Molecule details ›
Chains: F, R
Length: 575 amino acids
Theoretical weight: 64.16 KDa
Source organism: Mycolicibacterium smegmatis MC2 51
Probable cytochrome c oxidase subunit 3 Chains: G, S
Cytochrome c oxidase polypeptide 4 Chains: H, T
Cytochrome c oxidase subunit CtaJ Chains: I, U
Uncharacterized protein MSMEG_4692/MSMEI_4575 Chains: J, V
Prokaryotic respiratory supercomplex associate factor 1 PRSAF1 Chains: D, P
Cytochrome bc1 complex cytochrome b subunit Chains: B, N
Cytochrome bc1 complex Rieske iron-sulfur subunit Chains: A, M
Cytochrome bc1 complex cytochrome c subunit Chains: C, O

Ligands and Environments

11 bound ligands:
Ligand CU 8 x CU
Ligand CDL 17 x CDL
Ligand PLM 4 x PLM
Ligand HEA 4 x HEA
Ligand 9Y0 2 x 9Y0
Ligand HEM 4 x HEM
Ligand MQ9 8 x MQ9
Ligand HUU 2 x HUU
Ligand FES 2 x FES
Ligand 9YF 4 x 9YF
Ligand HEC 4 x HEC
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 2.67Å
Relevant EMDB volumes: EMD-30944
Expression system: Mycolicibacterium smegmatis MC2 51

Quick links

Citations

6 review citations

Antibiotics in the clinical pipeline as of December 2022.
Butler et al. (2023)
5 more

3 mentions without citation

Quinone binding sites of cyt bc complexes analysed by X-ray crystallography and cryogenic electron microscopy.
Kao et al. (2022)
2 more