› 7e2i

Electron Microscopy
4.07Å resolution

Cryo-EM structure of hDisp1NNN-ShhN

Released:
DOI: 10.2210/pdb7e2i/pdb
PDB entry 7e2i coloured by chain, front view.
PDB entry 7e2i coloured by chain, side view.
PDB entry 7e2i coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into proteolytic activation of the human Dispatched1 transporter for Hedgehog morphogen release.
Li W, Wang L, Wierbowski BM, Lu M, Dong F, Liu W, Li S, Wang P, Salic A, Gong X
Nat Commun 12 6966 (2021)
PMID: 34845226
Related structures: EMD-30958

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172298 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Sonic hedgehog protein Chain: G
Molecule details ›
Chain: G
Length: 462 amino acids
Theoretical weight: 49.68 KDa
Source organism: Homo sapiens
Expression system: Mammalian expression vector Flag-EGFP-MCS-pcDNA3.1
UniProt:
  • Canonical: Q15465 (Residues: 1-462; Coverage: 100%)
Gene name: SHH
Sequence domains:
Protein dispatched homolog 1 Chain: D
Molecule details ›
Chain: D
Length: 1524 amino acids
Theoretical weight: 171.11 KDa
Source organism: Homo sapiens
Expression system: Mammalian expression vector Flag-EGFP-MCS-pcDNA3.1
UniProt:
  • Canonical: Q96F81 (Residues: 1-1524; Coverage: 100%)
Gene names: DISP1, DISPA
Sequence domains: Patched family

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand Y01 7 x Y01
Ligand NAG 4 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.07Å
Relevant EMDB volumes: EMD-30958
Expression system: Mammalian expression vector Flag-EGFP-MCS-pcDNA3.1

Quick links

Citations

2 review citations

Cellular and molecular mechanisms of Hedgehog signalling.
Zhang et al. (2023)
1 more