› 7e31

X-ray diffraction
1.38Å resolution

Crystal structure of a novel alpha/beta hydrolase mutant in apo form

Released:
DOI: 10.2210/pdb7e31/pdb
PDB entry 7e31 coloured by chain, front view.
PDB entry 7e31 coloured by chain, side view.
PDB entry 7e31 coloured by chain, top view.
Source organism: unidentified
Primary publication:
Multiple Substrate Binding Mode-Guided Engineering of a Thermophilic PET Hydrolase.
Pfaff L, Gao J, Li Z, Jäckering A, Weber G, Mican J, Chen Y, Dong W, Han X, Feiler CG, Ao YF, Badenhorst CPS, Bednar D, Palm GJ, Lammers M, Damborsky J, Strodel B, Liu W, Bornscheuer UT, Wei R
ACS Catal 12 9790-9800 (2022)
PMID: 35966606

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-168193 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
alpha/beta hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 258 amino acids
Theoretical weight: 27.93 KDa
Source organism: unidentified
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

1 bound ligand:
Ligand PGE 2 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 55.343Å b: 94.865Å c: 105.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.165 0.175
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Microbial Enzyme Biotechnology to Reach Plastic Waste Circularity: Current Status, Problems and Perspectives.
Orlando et al. (2023)
3 more

1 mention without citation

Multiple Substrate Binding Mode-Guided Engineering of a Thermophilic PET Hydrolase.
Pfaff et al. (2022)