› 7e3q

X-ray diffraction
2.7Å resolution

Crystal structure of SAH bound TrmL from Vibrio vulnificus

Released:
DOI: 10.2210/pdb7e3q/pdb
Model geometry
Fit model/data
PDB entry 7e3q coloured by chain, front view.
PDB entry 7e3q coloured by chain, side view.
PDB entry 7e3q coloured by chain, top view.
Source organism: Vibrio vulnificus
Entry authors: Kim J, Son J

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + 5-carboxymethylaminomethyluridine(34) in tRNA(Leu) = S-adenosyl-L-homocysteine + 5-carboxymethylaminomethyl-2'-O-methyluridine(34) in tRNA(Leu)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181722 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA (cytidine(34)-2'-O)-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 165 amino acids
Theoretical weight: 18.83 KDa
Source organism: Vibrio vulnificus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7MGW0 (Residues: 1-157; Coverage: 100%)
Gene names: VV3116, trmL
Sequence domains: SpoU rRNA Methylase family

Ligands and Environments


Cofactor: Ligand SAH 1 x SAH
No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PAL/PLS BEAMLINE 11C
Spacegroup: P43212
Unit cell:
a: 67.354Å b: 67.354Å c: 145.611Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.189 0.252
Expression system: Escherichia coli BL21(DE3)

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