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7e4k

X-ray diffraction
2.69Å resolution

WNV envelope protein

Released:
Model geometry
Fit model/data
Source organism: West Nile virus
Entry authors: Xiao H, Qi J, Huang C, Song J, Cheng H

Function and Biology Details

Reactions catalysed:
a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleosidein mRNA + S-adenosyl-L-homocysteine.
a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA +S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleoside) in mRNA + S-adenosyl-L-homocysteine + H(+).
RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate.
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaacan be either Arg or Lys and Yaa can be either Ser or Ala.
a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate+ phosphate + H(+).
ATP + H2O = ADP + phosphate + H(+).
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-192974 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Envelope protein E Chain: A
Molecule details ›
Chain: A
Length: 404 amino acids
Theoretical weight: 43.63 KDa
Source organism: West Nile virus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Q6P4 (Residues: 291-693; Coverage: 12%)
Gene names: GP1, MZ11_60484gpGP1, MZ11_60553gpGP1
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P21
Unit cell:
a: 74.795Å b: 34.93Å c: 93.672Å
α: 90° β: 112.994° γ: 90°
R-values:
R R work R free
0.262 0.26 0.288
Expression system: Escherichia coli