7eal

X-ray diffraction
2.5Å resolution

The structure of the A20-Binding Inhibitor of NF-kB 1 in complex with di-ubiquitin

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-143376 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin Chains: A, D
Molecule details ›
Chains: A, D
Length: 152 amino acids
Theoretical weight: 17.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG48 (Residues: 533-684; Coverage: 22%)
Gene name: UBC
Sequence domains: Ubiquitin family
TNFAIP3-interacting protein 1 Chains: B, C, E, F
Molecule details ›
Chains: B, C, E, F
Length: 109 amino acids
Theoretical weight: 12.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15025 (Residues: 405-513; Coverage: 17%)
Gene names: KIAA0113, NAF1, TNIP1

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL15A1
Spacegroup: P21
Unit cell:
a: 80.753Å b: 59.99Å c: 84.058Å
α: 90° β: 109.71° γ: 90°
R-values:
R R work R free
0.186 0.184 0.224
Expression system: Escherichia coli