› 7ear

X-ray diffraction
2.2Å resolution

A positively charged mutant Cry3Aa endotoxin

Released:
DOI: 10.2210/pdb7ear/pdb
PDB entry 7ear coloured by chain, front view.
PDB entry 7ear coloured by chain, side view.
PDB entry 7ear coloured by chain, top view.
Source organism: Bacillus thuringiensis
Primary publication:
Efficient intracellular delivery of p53 protein by engineered protein crystals restores tumor suppressing function in vivo.
Yang Z, Lee MMM, Chan MK
Biomaterials 271 120759 (2021)
PMID: 33798968

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193414 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Crystaline entomocidal protoxin Chain: A
Molecule details ›
Chain: A
Length: 644 amino acids
Theoretical weight: 73.29 KDa
Source organism: Bacillus thuringiensis
Expression system: Bacillus thuringiensis
UniProt:
  • Canonical: Q9S6N9 (Residues: 9-652; Coverage: 99%)
Gene name: cry3Aa
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: C2221
Unit cell:
a: 114.84Å b: 132.89Å c: 103.13Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.216
Expression system: Bacillus thuringiensis

Quick links

Citations

1 review citation

Advances in immunotherapy for triple-negative breast cancer.
Liu et al. (2023)
2 other articles