› 7ebo

X-ray diffraction
2.5Å resolution

Crystal structure of a feruloyl esterase LP_0796 from Lactobacillus plantarum

Released:
DOI: 10.2210/pdb7ebo/pdb
PDB entry 7ebo coloured by chain, front view.
PDB entry 7ebo coloured by chain, side view.
PDB entry 7ebo coloured by chain, top view.
Source organism: Lactiplantibacillus plantarum WCFS1
Primary publication:
A reverse catalytic triad Asp containing loop shaping a wide substrate binding pocket of a feruloyl esterase from Lactobacillus plantarum.
Zhang H, Wen B, Liu Y, Du G, Wei X, Imam KMSU, Zhou H, Fan S, Wang F, Wang Y, Xin F
Int J Biol Macromol 184 92-100 (2021)
PMID: 34116094

Function and Biology Details

Reaction catalysed: 
A carboxylic ester + H(2)O = an alcohol + a carboxylate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123771 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine aminopeptidase S33 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 249 amino acids
Theoretical weight: 27.95 KDa
Source organism: Lactiplantibacillus plantarum WCFS1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F9UM18 (Residues: 1-249; Coverage: 100%)
Gene name: lp_0796
Sequence domains: Serine aminopeptidase, S33

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P212121
Unit cell:
a: 54.891Å b: 74.339Å c: 110.726Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.239
Expression system: Escherichia coli BL21(DE3)

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Citations

3 citation in other articles

Crystal structure of the feruloyl esterase from Lentilactobacillus buchneri reveals a novel homodimeric state.
Kasmaei et al. (2022)
2 more