› 7ebp

X-ray diffraction
1.8Å resolution

The structural analysis of A.Muciniphila sulfatase

Released:
DOI: 10.2210/pdb7ebp/pdb
Model geometry
Fit model/data
PDB entry 7ebp coloured by chain, front view.
PDB entry 7ebp coloured by chain, side view.
PDB entry 7ebp coloured by chain, top view.
Source organism: Akkermansia muciniphila ATCC BAA-835
Primary publication:
Structural analysis of the sulfatase AmAS from Akkermansia muciniphila.
Li CC, Tang XY, Zhu YB, Song YJ, Zhao NL, Huang Q, Mou XY, Luo GH, Liu TG, Tong AP, Tang H, Bao R
Acta Crystallogr D Struct Biol 77 1614-1623 (2021)
PMID: 34866616

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109744 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-sulphoglucosamine sulphohydrolase C-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 530 amino acids
Theoretical weight: 60.73 KDa
Source organism: Akkermansia muciniphila ATCC BAA-835
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B2UR15 (Residues: 23-552; Coverage: 100%)
Gene name: Amuc_1074
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P212121
Unit cell:
a: 64.342Å b: 112.722Å c: 165.011Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.186 0.209
Expression system: Escherichia coli BL21(DE3)

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Citations

2 citation in other articles

Health and Disease: Akkermansia muciniphila, the Shining Star of the Gut Flora.
Xue et al. (2023)
1 more