› 7ecb

X-ray diffraction
1.83Å resolution

Polyethylene terephthalate hydrolyzing lipase PET2 mutant - R47C-G89C-F105R-E110K-S156P-G180A-T297P

Released:
DOI: 10.2210/pdb7ecb/pdb
PDB entry 7ecb coloured by chain, front view.
PDB entry 7ecb coloured by chain, side view.
PDB entry 7ecb coloured by chain, top view.
Source organism: uncultured bacterium
Primary publication:
Positive Charge Introduction on the Surface of Thermostabilized PET Hydrolase Facilitates PET Binding and Degradation.
Nakamura A
ACS Catalysis (2021)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111179 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dienelactone hydrolase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 294 amino acids
Theoretical weight: 31.62 KDa
Source organism: uncultured bacterium
Expression system: Escherichia coli
UniProt:
  • Canonical: C3RYL0 (Residues: 28-308; Coverage: 100%)
Gene name: lipIAF5-2
Sequence domains: Dienelactone hydrolase family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AichiSR BEAMLINE BL2S1
Spacegroup: P21212
Unit cell:
a: 67.24Å b: 86.72Å c: 46.21Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.15 0.192
Expression system: Escherichia coli

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