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› 7egh

Electron Microscopy
3.04Å resolution

TFIID lobe C subcomplex

Released:
DOI: 10.2210/pdb7egh/pdb
PDB entry 7egh coloured by chain, front view.
PDB entry 7egh coloured by chain, side view.
PDB entry 7egh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into preinitiation complex assembly on core promoters.
Chen X, Qi Y, Wu Z, Wang X, Li J, Zhao D, Hou H, Li Y, Yu Z, Liu W, Wang M, Ren Y, Li Z, Yang H, Xu Y
Science 372 (2021)
PMID: 33795473
Related structures: EMD-31117

Function and Biology Details

Reactions catalysed: 
L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA +H(+).
(1) L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-121252 (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (7 distinct):
Transcription initiation factor TFIID subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 1872 amino acids
Theoretical weight: 212.96 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P21675 (Residues: 1-1893; Coverage: 99%)
  • Best match: P21675-1 (Residues: 1-1872)
Gene names: BA2R, CCG1, CCGS, TAF1, TAF2A
Sequence domains:
Transcription initiation factor TFIID subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 1199 amino acids
Theoretical weight: 137.16 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q6P1X5 (Residues: 1-1199; Coverage: 100%)
Gene names: CIF150, TAF2, TAF2B
Transcription initiation factor TFIID subunit 6 Chains: F, f
Transcription initiation factor TFIID subunit 7 Chain: G
Transcription initiation factor TFIID subunit 8 Chain: H
DNA (45-MER) Chain: X
DNA (45-MER) Chain: Y

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.04Å
Relevant EMDB volumes: EMD-31117
Expression system: Homo sapiens

Quick links

Citations

17 review citations

The Mediator complex as a master regulator of transcription by RNA polymerase II.
Richter et al. (2022)
16 more

2 mentions without citation

Hierarchical TAF1-dependent co-translational assembly of the basal transcription factor TFIID.
Bernardini et al. (2023)
1 more