› 7enq

X-ray diffraction
2.2Å resolution

Crystal structure of human NAMPT in complex with compound NAT

Released:
DOI: 10.2210/pdb7enq/pdb
Model geometry
Fit model/data
PDB entry 7enq coloured by chain, front view.
PDB entry 7enq coloured by chain, side view.
PDB entry 7enq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Discovery of small-molecule activators of nicotinamide phosphoribosyltransferase (NAMPT) and their preclinical neuroprotective activity.
Yao H, Liu M, Wang L, Zu Y, Wu C, Li C, Zhang R, Lu H, Li F, Xi S, Chen S, Gu X, Liu T, Cai J, Wang S, Yang M, Xing GG, Xiong W, Hua L, Tang Y, Wang G
Cell Res 32 570-584 (2022)
PMID: 35459935

Function and Biology Details

Reaction catalysed: 
Nicotinamide D-ribonucleotide + diphosphate = nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155082 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nicotinamide phosphoribosyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 497 amino acids
Theoretical weight: 56.42 KDa
Source organism: Homo sapiens
Expression system: Expression vector pET-mod
UniProt:
  • Canonical: P43490 (Residues: 1-491; Coverage: 100%)
Gene names: NAMPT, PBEF, PBEF1
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand J7F 4 x J7F
Ligand PO4 6 x PO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P212121
Unit cell:
a: 86.241Å b: 93.627Å c: 242.614Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.178 0.235
Expression system: Expression vector pET-mod

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Citations

4 review citations

Targeting NAD Metabolism for the Therapy of Age-Related Neurodegenerative Diseases.
Li et al. (2023)
3 more

1 mention without citation

Discovery of small-molecule activators of nicotinamide phosphoribosyltransferase (NAMPT) and their preclinical neuroprotective activity.
Yao et al. (2022)