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7eqg

Electron Microscopy
3.2Å resolution

Structure of Csy-AcrIF5

Released:
Source organism: Pseudomonas aeruginosa
Related structures: EMD-31265

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero heptadecamer (preferred)
PDBe Complex ID:
PDB-CPX-215585 (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (8 distinct):
CRISPR-associated protein Csy2 Chain: C
Molecule details ›
Chain: C
Length: 327 amino acids
Theoretical weight: 36.24 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02MM0 (Residues: 1-327; Coverage: 100%)
Gene names: PA14_33320, csy2
Sequence domains: CRISPR-associated protein (Cas_Csy2)
CRISPR-associated protein Csy3 Chains: D, E, F, G, H, I
Molecule details ›
Chains: D, E, F, G, H, I
Length: 342 amino acids
Theoretical weight: 37.62 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02MM1 (Residues: 1-342; Coverage: 100%)
Gene names: PA14_33310, csy1-3, csy3
Sequence domains: CRISPR-associated protein (Cas_Csy3)
DRBM domain-containing protein Chains: J, K, P, Q, R
CRISPR-associated endonuclease Cas6/Csy4 Chain: L
Type I-F CRISPR-associated protein Csy1 Chain: B
DNA (54-MER) Chain: N
DNA (54-MER) Chain: O
RNA (60-MER) Chain: M

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.2Å
Relevant EMDB volumes: EMD-31265
Expression system: Escherichia coli