› 7evd

X-ray diffraction
1.45Å resolution

Odinarchaeota tubulin (OdinTubulin) H393D mutant, in a protofilament arrangement, bound to 53% GTP/47% and 2 Na+

Released:
DOI: 10.2210/pdb7evd/pdb
Model geometry
Fit model/data
PDB entry 7evd coloured by chain, front view.
PDB entry 7evd coloured by chain, side view.
PDB entry 7evd coloured by chain, top view.
Source organism: Candidatus Odinarchaeota archaeon LCB_4
Primary publication:
Structure and dynamics of Odinarchaeota tubulin and the implications for eukaryotic microtubule evolution.
Akıl C, Ali S, Tran LT, Gaillard J, Li W, Hayashida K, Hirose M, Kato T, Oshima A, Fujishima K, Blanchoin L, Narita A, Robinson RC
OpenAccess logo Sci Adv 8 eabm2225 (2022)
PMID: 35333570

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-245362 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tubulin-like protein Chain: B
Molecule details ›
Chain: B
Length: 425 amino acids
Theoretical weight: 47.63 KDa
Source organism: Candidatus Odinarchaeota archaeon LCB_4
Expression system: Escherichia coli

Ligands and Environments

3 bound ligands:
Ligand GTP 1 x GTP
Ligand GDP 1 x GDP
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSRRC BEAMLINE TPS 05A
Spacegroup: P212121
Unit cell:
a: 40.361Å b: 92.104Å c: 102.824Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.152 0.176
Expression system: Escherichia coli

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Citations

2 review citations

Alternative Approaches to Understand Microtubule Cap Morphology and Function.
Oliva et al. (2023)
1 more