› 7evr

X-ray diffraction
1.8Å resolution

Crystal structure of hnRNP L RRM2 in complex with SETD2

Released:
DOI: 10.2210/pdb7evr/pdb
Model geometry
Fit model/data
PDB entry 7evr coloured by chain, front view.
PDB entry 7evr coloured by chain, side view.
PDB entry 7evr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of the interaction between SETD2 methyltransferase and hnRNP L paralogs for governing co-transcriptional splicing.
Bhattacharya S, Wang S, Reddy D, Shen S, Zhang Y, Zhang N, Li H, Washburn MP, Florens L, Shi Y, Workman JL, Li F
OpenAccess logo Nat Commun 12 6452 (2021)
PMID: 34750379

Function and Biology Details

Reaction catalysed: 
(1a) S-adenosyl-L-methionine + a [histone H3]-L-lysine(36) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(36)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147108 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Heterogeneous nuclear ribonucleoprotein L Chains: A, C
Molecule details ›
Chains: A, C
Length: 111 amino acids
Theoretical weight: 12.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14866 (Residues: 186-289; Coverage: 18%)
Gene names: HNRNPL, HNRPL, P/OKcl.14
Sequence domains: RRM-like domain
Histone-lysine N-methyltransferase SETD2 Chains: B, D
Molecule details ›
Chains: B, D
Length: 26 amino acids
Theoretical weight: 2.79 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9BYW2 (Residues: 2167-2192; Coverage: 1%)
Gene names: HIF1, HSPC069, HYPB, KIAA1732, KMT3A, SET2, SETD2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21
Unit cell:
a: 56.776Å b: 38.789Å c: 68.982Å
α: 90° β: 100.31° γ: 90°
R-values:
R R work R free
0.16 0.158 0.198
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Histone methyltransferase SETD2: An epigenetic driver in clear cell renal cell carcinoma.
Yu et al. (2023)
7 other articles

1 mention without citation

A novel SETD2 variant causing global development delay without overgrowth in a Chinese 3-year-old boy.
Wu et al. (2023)