Function and Biology Details
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-131731 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Protein THP3
Molecule details ›
Chain: A
Length: 289 amino acids
Theoretical weight: 33.96 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: SAC3/GANP family
Length: 289 amino acids
Theoretical weight: 33.96 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
Q12049 (Residues: 186-470; Coverage: 61%)
Sequence domains: SAC3/GANP family
Cop9 signalosome complex subunit 12
Molecule details ›
Chain: B
Length: 423 amino acids
Theoretical weight: 49.56 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: PCI domain
Length: 423 amino acids
Theoretical weight: 49.56 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P47130 (Residues: 1-423; Coverage: 100%)
Sequence domains: PCI domain
26S proteasome complex subunit SEM1
Molecule details ›
Chain: C
Length: 89 amino acids
Theoretical weight: 10.4 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: DSS1/SEM1 family
Length: 89 amino acids
Theoretical weight: 10.4 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: O94742 (Residues: 1-89; Coverage: 100%)
Sequence domains: DSS1/SEM1 family
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
SSRF BEAMLINE BL18U1
Spacegroup:
P3221
Expression system: Escherichia coli BL21(DE3)
