› 7ezj

X-ray diffraction
2.9Å resolution

Crystal structure of p73 DNA binding domain complex bound with 1 bp and 2 bp spacer DNA response elements.

Released:
DOI: 10.2210/pdb7ezj/pdb
PDB entry 7ezj coloured by chain, front view.
PDB entry 7ezj coloured by chain, side view.
PDB entry 7ezj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Deciphering the mechanism of p73 recognition of p53 response elements using the crystal structure of p73-DNA complexes and computational studies.
Koley T, Chowdhury SR, Kushwaha T, Kumar M, Inampudi KK, Kaur P, Singh TP, Viadiu H, Ethayathulla AS
Int J Biol Macromol 206 40-50 (2022)
PMID: 35217090

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-121272 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Tumor protein p73 Chains: A, B, C, D, I, J, K, L, a, b, c, d, i, j, k, l
Molecule details ›
Chains: A, B, C, D, I, J, K, L, a, b, c, d, i, j, k, l
Length: 210 amino acids
Theoretical weight: 23.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O15350 (Residues: 115-312; Coverage: 31%)
Gene names: P73, TP73
Sequence domains: P53 DNA-binding domain
12-mer DNA Chains: E, F, G, H, M, N, O, P, e, f, g, h, m, n, o, p
Molecule details ›
Chains: E, F, G, H, M, N, O, P, e, f, g, h, m, n, o, p
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 16 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21
Unit cell:
a: 139.697Å b: 105.496Å c: 155.504Å
α: 90° β: 112.36° γ: 90°
R-values:
R R work R free
0.244 0.241 0.306
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

1 mention without citation

The Nucleic Acid Knowledgebase: a new portal for 3D structural information about nucleic acids.
Lawson et al. (2024)