› 7f2e

X-ray diffraction
3.1Å resolution

SARS-CoV-2 nucleocapsid protein C-terminal domain (dodecamer)

Released:
DOI: 10.2210/pdb7f2e/pdb
Model geometry
Fit model/data
PDB entry 7f2e coloured by chain, front view.
PDB entry 7f2e coloured by chain, side view.
PDB entry 7f2e coloured by chain, top view.
Source organism: Severe acute respiratory syndrome coronavirus 2
Primary publication:
Crystal structures of the SARS-CoV-2 nucleocapsid protein C-terminal domain and development of nucleocapsid-targeting nanobodies.
Jia Z, Liu C, Chen Y, Jiang H, Wang Z, Yao J, Yang J, Zhu J, Zhang B, Yuchi Z
OpenAccess logo FEBS J 289 3813-3825 (2022)
PMID: 34665939

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145015 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleoprotein Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 108 amino acids
Theoretical weight: 12.23 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DTC9 (Residues: 255-362; Coverage: 26%)
Gene name: N

Ligands and Environments

1 bound ligand:
Ligand PO4 4 x PO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: R3
Unit cell:
a: 102.542Å b: 102.542Å c: 389.677Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.269 0.268 0.298
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

SARS-CoV-2 variants preferentially emerge at intrinsically disordered protein sites helping immune evasion.
Quaglia et al. (2022)
2 more

3 mentions without citation

Crystal structures of the SARS-CoV-2 nucleocapsid protein C-terminal domain and development of nucleocapsid-targeting nanobodies.
Jia et al. (2022)
2 more