› 7f5w

X-ray diffraction
1.65Å resolution

Conserved and divergent strigolactone signaling in Saccharum spontaneum

Released:
DOI: 10.2210/pdb7f5w/pdb
PDB entry 7f5w coloured by chain, front view.
PDB entry 7f5w coloured by chain, side view.
PDB entry 7f5w coloured by chain, top view.
Source organism: Saccharum hybrid cultivar ROC22
Primary publication:
Identification of Conserved and Divergent Strigolactone Receptors in Sugarcane Reveals a Key Residue Crucial for Plant Branching Control.
Hu A, Zhao Q, Chen L, Zhao J, Wang Y, Feng K, Wu L, Xie M, Zhou X, Xiao L, Ming Z, Zhang M, Yao R
Front Plant Sci 12 747160 (2021)
PMID: 34858455

Function and Biology Details

Reaction catalysed: 
2-lysophosphatidylcholine + H(2)O = glycerophosphocholine + a carboxylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101118 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 308 amino acids
Theoretical weight: 32.81 KDa
Source organism: Saccharum hybrid cultivar ROC22
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A0D5NT23 (Residues: 1-308; Coverage: 100%)
Gene name: HTD2
Sequence domains: Alpha/beta hydrolase family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P212121
Unit cell:
a: 48.81Å b: 88.295Å c: 118.521Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.191 0.228
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

2 mentions without citation

Identification of Conserved and Divergent Strigolactone Receptors in Sugarcane Reveals a Key Residue Crucial for Plant Branching Control.
Hu et al. (2021)
1 more