› 7f8k

X-ray diffraction
2.2Å resolution

Room temperature structure of bacterial copper amine oxidase determined by serial femtosecond crystallography

Released:
DOI: 10.2210/pdb7f8k/pdb
PDB entry 7f8k coloured by chain, front view.
PDB entry 7f8k coloured by chain, side view.
PDB entry 7f8k coloured by chain, top view.
Source organism: Arthrobacter globiformis
Primary publication:
Microcrystal preparation for serial femtosecond X-ray crystallography of bacterial copper amine oxidase.
Murakawa T, Suzuki M, Arima T, Sugahara M, Tanaka T, Tanaka R, Iwata S, Nango E, Tono K, Hayashi H, Fukui K, Yano T, Tanizawa K, Okajima T
Acta Crystallogr F Struct Biol Commun 77 356-363 (2021)
PMID: 34605440

Function and Biology Details

Reaction catalysed: 
RCH(2)NH(2) + H(2)O + O(2) = RCHO + NH(3) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155526 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenylethylamine oxidase Chain: A
Molecule details ›
Chain: A
Length: 638 amino acids
Theoretical weight: 70.75 KDa
Source organism: Arthrobacter globiformis
Expression system: Escherichia coli
UniProt:
  • Canonical: P46881 (Residues: 1-638; Coverage: 100%)
Sequence domains: Copper amine oxidase, enzyme domain

Ligands and Environments

1 bound ligand:
Ligand CU 1 x CU
1 modified residue:
Ligand TPQ 1 x TPQ

Experiments and Validation Details

Entry percentile scores
X-ray source: SACLA BEAMLINE BL2
Spacegroup: C2
Unit cell:
a: 158.75Å b: 64.74Å c: 93.55Å
α: 90° β: 112.33° γ: 90°
R-values:
R R work R free
0.156 0.154 0.179
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Growing and making nano- and microcrystals.
Shoeman et al. (2023)

1 mention without citation

Microcrystal preparation for serial femtosecond X-ray crystallography of bacterial copper amine oxidase.
Murakawa et al. (2021)