› 7fax

X-ray diffraction
1.8Å resolution

Complex structure of TbLeo1 and LW domain from Trypanosoma brucei

Released:
DOI: 10.2210/pdb7fax/pdb
PDB entry 7fax coloured by chain, front view.
PDB entry 7fax coloured by chain, side view.
PDB entry 7fax coloured by chain, top view.
Source organism: Trypanosoma brucei brucei TREU927
Primary publication:
Structural basis for evolutionarily conserved interactions between TFIIS and Paf1C.
Gao J, Jishage M, Wang Y, Wang R, Chen M, Zhu Z, Zhang J, Diwu Y, Xu C, Liao S, Roeder RG, Tu X
Int J Biol Macromol 253 126764 (2023)
PMID: 37696373

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174410 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
PWWP domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 111 amino acids
Theoretical weight: 12.41 KDa
Source organism: Trypanosoma brucei brucei TREU927
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q586Y0 (Residues: 251-358; Coverage: 28%)
Gene names: 28H13.90, Tb927.2.3480
Sequence domains: TFIIS helical bundle-like domain
RNA polymerase-associated protein LEO1 Chain: B
Molecule details ›
Chain: B
Length: 18 amino acids
Theoretical weight: 2 KDa
Source organism: Trypanosoma brucei brucei TREU927
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q38DC5 (Residues: 71-86; Coverage: 3%)
Gene name: Tb09.211.3840

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: I4
Unit cell:
a: 79.919Å b: 79.919Å c: 38.843Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.215 0.238
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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