› 7fbw

X-ray diffraction
1.9Å resolution

Acetylxylan esterase from Caldanaerobacter subterraneus subsp. tengcongensis

Released:
DOI: 10.2210/pdb7fbw/pdb
PDB entry 7fbw coloured by chain, front view.
PDB entry 7fbw coloured by chain, side view.
PDB entry 7fbw coloured by chain, top view.
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis MB4
Primary publication:
Crystal structure of acetylxylan esterase from Caldanaerobacter subterraneus subsp. tengcongensis.
Sasamoto K, Himiyama T, Moriyoshi K, Ohmoto T, Uegaki K, Nishiya Y, Nakamura T
Acta Crystallogr F Struct Biol Commun 77 399-406 (2021)
PMID: 34726178

Function and Biology Details

Reaction catalysed: 
Peptidoglycan-N-acetyl-D-glucosamine + H(2)O = peptidoglycan-D-glucosamine + acetate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185946 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
peptidoglycan-N-acetylglucosamine deacetylase Chain: A
Molecule details ›
Chain: A
Length: 304 amino acids
Theoretical weight: 34.56 KDa
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis MB4
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8RBF4 (Residues: 23-324; Coverage: 93%)
Gene names: Cda1, TTE0866
Sequence domains: Polysaccharide deacetylase

Ligands and Environments

1 bound ligand:
Ligand NI 1 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: I212121
Unit cell:
a: 62.211Å b: 105.992Å c: 118.077Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.186 0.21
Expression system: Escherichia coli

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Citations

2 mentions without citation

Functional analysis of the N-terminal region of acetylxylan esterase from Caldanaerobacter subterraneus subsp. tengcongensis.
Sasamoto et al. (2022)
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