› 7fcp

X-ray diffraction
2.4Å resolution

Crystallographic structure of two neutralizing antibodies in complex with SARS-CoV-2 spike receptor-binding Domain (RBD)

Released:
DOI: 10.2210/pdb7fcp/pdb
Model geometry
Fit model/data
PDB entry 7fcp coloured by chain, front view.
PDB entry 7fcp coloured by chain, side view.
PDB entry 7fcp coloured by chain, top view.
Source organisms:
Primary publication:
Ultrapotent neutralizing antibodies against SARS-CoV-2 with a high degree of mutation resistance.
Zou J, Li L, Zheng P, Liang W, Hu S, Zhou S, Wang Y, Zhao J, Yuan D, Liu L, Wu D, Xu M, Zhang F, Zhu M, Wu Z, Cao X, Ni M, Ling X, Wu Y, Kuang Z, Hu M, Li J, Li X, Guo X, Xu T, Jiang H, Gao C, Yu M, Liu J, Zhong N, Zhou J, Huang JA, Jin T, He J
OpenAccess logo J Clin Invest 132 (2022)
PMID: 35108220

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-279647 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Spike protein S1 Chain: A
Molecule details ›
Chain: A
Length: 277 amino acids
Theoretical weight: 30.94 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Homo sapiens
UniProt:
  • Canonical: P0DTC2 (Residues: 321-591; Coverage: 22%)
Gene names: 2, S
Sequence domains:
P14-44 antibody Fab fragment heavy chain Chain: H
Molecule details ›
Chain: H
Length: 235 amino acids
Theoretical weight: 25.48 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
P14-44 antibody Fab fragment light chain Chain: L
P5-22 antibody Fab fragment heavy chain Chain: B
P5-22 antibody Fab fragment light chain Chain: D

Ligands and Environments

3 bound ligands:
Ligand TRS 1 x TRS
Ligand NAG 1 x NAG
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NFPSS BEAMLINE BL19U1
Spacegroup: C2
Unit cell:
a: 223.007Å b: 80.414Å c: 72.746Å
α: 90° β: 106.23° γ: 90°
R-values:
R R work R free
0.194 0.191 0.245
Expression system: Homo sapiens

Quick links

Citations

5 review citations

Research and development of Chinese anti-COVID-19 drugs.
Ji et al. (2022)
4 more

1 mention without citation

Atomically accurate de novo design of single-domain antibodies.
Bennett et al. (2024)