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› 7fhd

X-ray diffraction
3.5Å resolution

Structure of prenyltransferase mutant Y288P from Streptomyces sp. (strain CL190)

Released:
DOI: 10.2210/pdb7fhd/pdb
Model geometry
Fit model/data
PDB entry 7fhd coloured by chain, front view.
PDB entry 7fhd coloured by chain, side view.
PDB entry 7fhd coloured by chain, top view.
Source organism: Streptomyces sp. CL190
Primary publication:
Structure-Guided Engineering of Prenyltransferase NphB for High-Yield and Regioselective Cannabinoid Production.
Lim KJH, Hartono YD, Xue B, Go MK, Fan H, Yew WS
ACS Catalysis 12 4628-4639 (2022)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175745 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Prenyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 309 amino acids
Theoretical weight: 33.87 KDa
Source organism: Streptomyces sp. CL190
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4R2T2 (Residues: 1-307; Coverage: 100%)
Sequence domains: Aromatic prenyltransferase Orf2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P1
Unit cell:
a: 44.286Å b: 75.162Å c: 87.605Å
α: 89.939° β: 89.903° γ: 89.996°
R-values:
R R work R free
0.247 0.243 0.313
Expression system: Escherichia coli BL21(DE3)

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