› 7fix

Electron Microscopy
1.97Å resolution

Cryo-EM structure of cyanobacterial photosystem I in the presence of ferredoxin and cytochrome c6

Released:
DOI: 10.2210/pdb7fix/pdb
PDB entry 7fix coloured by chain, front view.
PDB entry 7fix coloured by chain, side view.
PDB entry 7fix coloured by chain, top view.
Source organism: Thermosynechococcus vestitus BP-1
Primary publication:
Structure of cyanobacterial photosystem I complexed with ferredoxin at 1.97 Å resolution.
Li J, Hamaoka N, Makino F, Kawamoto A, Lin Y, Rögner M, Nowaczyk MM, Lee YH, Namba K, Gerle C, Kurisu G
OpenAccess logo Commun Biol 5 951 (2022)
PMID: 36097054
Related structures: EMD-31605

Function and Biology Details

Reaction catalysed: 
Reduced plastocyanin + oxidized ferredoxin + light = oxidized plastocyanin + reduced ferredoxin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 39-mer (preferred)
PDBe Complex ID:
PDB-CPX-141201 (preferred)
Entry contents:
13 distinct polypeptide molecules
Macromolecules (13 distinct):
Photosystem I P700 chlorophyll a apoprotein A1 Chains: A1, A2, A3
Molecule details ›
Chains: A1, A2, A3
Length: 755 amino acids
Theoretical weight: 83.27 KDa
Source organism: Thermosynechococcus vestitus BP-1
UniProt:
  • Canonical: P0A405 (Residues: 1-755; Coverage: 100%)
Gene names: psaA, tlr0731
Sequence domains: Photosystem I psaA/psaB protein
Photosystem I P700 chlorophyll a apoprotein A2 Chains: B1, B2, B3
Molecule details ›
Chains: B1, B2, B3
Length: 741 amino acids
Theoretical weight: 83.12 KDa
Source organism: Thermosynechococcus vestitus BP-1
UniProt:
  • Canonical: P0A407 (Residues: 1-741; Coverage: 100%)
Gene names: psaB, tlr0732
Sequence domains: Photosystem I psaA/psaB protein
Photosystem I iron-sulfur center Chains: C1, C2, C3
Photosystem I reaction center subunit II Chains: D1, D2, D3
Photosystem I reaction center subunit IV Chains: E1, E2, E3
Photosystem I reaction center subunit III Chains: F1, F2, F3
Photosystem I reaction center subunit VIII Chains: I1, I2, I3
Photosystem I reaction center subunit IX Chains: J1, J2, J3
Photosystem I reaction center subunit PsaK Chains: K1, K2, K3
Photosystem I reaction center subunit XI Chains: L1, L2, L3
Photosystem I reaction center subunit XII Chains: M1, M2, M3
Ferredoxin-1 Chains: R1, R2, R3
Photosystem I 4.8K protein Chains: X1, X2, X3

Ligands and Environments

10 bound ligands:
Ligand CL0 3 x CL0
Ligand CLA 285 x CLA
Ligand PQN 6 x PQN
Ligand SF4 9 x SF4
Ligand BCR 72 x BCR
Ligand LHG 9 x LHG
Ligand UNL 51 x UNL
Ligand DGD 3 x DGD
Ligand CA 3 x CA
Ligand GAK 3 x GAK
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 1.97Å
Relevant EMDB volumes: EMD-31605
Expression systems:
  • Thermosynechococcus vestitus BP-1
  • Escherichia coli

Quick links

Citations

1 review citation

Investigating the Balance between Structural Conservation and Functional Flexibility in Photosystem I.
Nelson N. (2024)
1 other article

1 mention without citation

Structure of cyanobacterial photosystem I complexed with ferredoxin at 1.97 Å resolution.
Li et al. (2022)