› 7jjd

X-ray diffraction
1.21Å resolution

Sarcin-ricin loop with guanosine monothiophosphate residue.

Released:
DOI: 10.2210/pdb7jjd/pdb
PDB entry 7jjd coloured by chain, front view.
PDB entry 7jjd coloured by chain, side view.
PDB entry 7jjd coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Incorporating a Thiophosphate Modification into a Common RNA Tetraloop Motif Causes an Unanticipated Stability Boost.
Pallan PS, Lybrand TP, Schlegel MK, Harp JM, Jahns H, Manoharan M, Egli M
Biochemistry 59 4627-4637 (2020)
PMID: 33275419

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197252 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
RNA (27-MER) Chain: A
Molecule details ›
Chain: A
Length: 27 nucleotides
Theoretical weight: 8.76 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand G46 1 x G46

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P43
Unit cell:
a: 29.639Å b: 29.639Å c: 76.658Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.173 0.239
Expression system: Not provided

Quick links

Citations

1 review citation

Beyond ribose and phosphate: Selected nucleic acid modifications for structure-function investigations and therapeutic applications.
Liczner et al. (2021)
2 other articles

1 mention without citation

Cryo neutron crystallography demonstrates influence of RNA 2'-OH orientation on conformation, sugar pucker and water structure.
Harp et al. (2022)