› 7jjm

X-ray diffraction
2.06Å resolution

Crystal structure of Importin alpha 2 in complex with LSD1 NLS

Released:
DOI: 10.2210/pdb7jjm/pdb
Model geometry
Fit model/data
PDB entry 7jjm coloured by chain, front view.
PDB entry 7jjm coloured by chain, side view.
PDB entry 7jjm coloured by chain, top view.
Source organisms:
Primary publication:
Targeting Nuclear LSD1 to Reprogram Cancer Cells and Reinvigorate Exhausted T Cells via a Novel LSD1-EOMES Switch.
Tu WJ, McCuaig RD, Tan AHY, Hardy K, Seddiki N, Ali S, Dahlstrom JE, Bean EG, Dunn J, Forwood J, Tsimbalyuk S, Smith K, Yip D, Malik L, Prasanna T, Milburn P, Rao S
Front Immunol 11 1228- (2020)

Function and Biology Details

Reaction catalysed: 
N(6),N(6)-dimethyl-L-lysyl(4)-[histone H3] + 2 A + 2 H2O = L-lysyl(4)-[histone H3] + 2 formaldehyde + 2 AH2.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129948 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Lysine-specific histone demethylase 1A Chain: A
Molecule details ›
Chain: A
Length: 26 amino acids
Theoretical weight: 3.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O60341 (Residues: 104-129; Coverage: 3%)
Gene names: AOF2, KDM1, KDM1A, KIAA0601, LSD1
Importin subunit alpha-1 Chain: B
Molecule details ›
Chain: B
Length: 470 amino acids
Theoretical weight: 50.97 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P52293 (Residues: 62-529; Coverage: 89%)
Gene names: Kpna2, Rch1
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 78.61Å b: 90.05Å c: 99.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.22 0.259
Expression system: Escherichia coli

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