› 7jk6

Electron Microscopy
4Å resolution

Structure of Drosophila ORC in the active conformation

Released:
DOI: 10.2210/pdb7jk6/pdb
PDB entry 7jk6 coloured by chain, front view.
PDB entry 7jk6 coloured by chain, side view.
PDB entry 7jk6 coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structural mechanism for replication origin binding and remodeling by a metazoan origin recognition complex and its co-loader Cdc6.
Schmidt JM, Bleichert F
OpenAccess logo Nat Commun 11 4263 (2020)
PMID: 32848132
Related structures: EMD-22363

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127568 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Origin recognition complex subunit 4 Chain: D
Molecule details ›
Chain: D
Length: 462 amino acids
Theoretical weight: 52.28 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9W102 (Residues: 1-459; Coverage: 100%)
Gene names: CG2917, DmORC4, Dmel\CG2917, Dmel_CG2917, ORC, ORC4, Orc4, dmOrc4, orc4, rDmORC
Sequence domains:
Origin recognition complex subunit 5 Chain: E
Molecule details ›
Chain: E
Length: 460 amino acids
Theoretical weight: 52.18 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q24169 (Residues: 1-460; Coverage: 100%)
Gene names: CG7833, Orc5
Sequence domains:
Origin recognition complex subunit 1 Chain: A
Origin recognition complex subunit 2 Chain: B
Origin recognition complex subunit 3 Chain: C
Origin recognition complex subunit 6 Chain: F

Ligands and Environments

2 bound ligands:
Ligand ATP 3 x ATP
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 4Å
Relevant EMDB volumes: EMD-22363
Expression system: Trichoplusia ni

Quick links

Citations

1 review citation

Replication initiation: Implications in genome integrity.
Lin et al. (2021)
16 other articles

1 mention without citation

Systemic analysis of the DNA replication regulator origin recognition complex in lung adenocarcinomas identifies prognostic and expression significance.
Tang et al. (2023)