› 7jn7

Electron Microscopy
3.3Å resolution

Human DPP9-CARD8 complex

Released:
DOI: 10.2210/pdb7jn7/pdb
PDB entry 7jn7 coloured by chain, front view.
PDB entry 7jn7 coloured by chain, side view.
PDB entry 7jn7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Dipeptidyl peptidase 9 sets a threshold for CARD8 inflammasome formation by sequestering its active C-terminal fragment.
Sharif H, Hollingsworth LR, Griswold AR, Hsiao JC, Wang Q, Bachovchin DA, Wu H
Immunity 54 1392-1404.e10 (2021)
PMID: 34019797
Related structures: EMD-22402

Function and Biology Details

Reaction catalysed: 
Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183167 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Dipeptidyl peptidase 9 Chains: A, D
Molecule details ›
Chains: A, D
Length: 891 amino acids
Theoretical weight: 101.76 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q86TI2 (Residues: 1-863; Coverage: 100%)
Gene names: DPP9, DPRP2
Sequence domains:
Caspase recruitment domain-containing protein 8 Chains: B, C
Molecule details ›
Chains: B, C
Length: 537 amino acids
Theoretical weight: 60.72 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9Y2G2 (Residues: 1-537; Coverage: 100%)
Gene names: CARD8, DACAR, KIAA0955, NDPP1
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand GK2 2 x GK2
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-22402
Expression system: Homo sapiens

Quick links

Citations

8 review citations

Genome-wide association studies of COVID-19: Connecting the dots.
Ferreira et al. (2022)
7 more

1 mention without citation

Dipeptidyl peptidase 9 sets a threshold for CARD8 inflammasome formation by sequestering its active C-terminal fragment.
Sharif et al. (2021)