› 7jps

Electron Microscopy
4.4Å resolution

ORC-DNA: Human Origin Recognition Complex (ORC) with DNA bound in the core

Released:
DOI: 10.2210/pdb7jps/pdb
PDB entry 7jps coloured by chain, front view.
PDB entry 7jps coloured by chain, side view.
PDB entry 7jps coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The dynamic nature of the human origin recognition complex revealed through five cryoEM structures.
Jaremko MJ, On KF, Thomas DR, Stillman B, Joshua-Tor L
Elife 9 (2020)
PMID: 32808929
Related structures: EMD-22421

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-121390 (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (7 distinct):
Origin recognition complex subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 392 amino acids
Theoretical weight: 44.31 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q13415 (Residues: 471-861; Coverage: 45%)
Gene names: ORC1, ORC1L, PARC1
Sequence domains:
Origin recognition complex subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 577 amino acids
Theoretical weight: 66.06 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q13416 (Residues: 1-577; Coverage: 100%)
Gene names: ORC2, ORC2L
Sequence domains: Origin recognition complex subunit 2
Origin recognition complex subunit 3 Chain: C
Molecule details ›
Chain: C
Length: 712 amino acids
Theoretical weight: 82.44 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9UBD5 (Residues: 1-505, 506-711; Coverage: 100%)
Gene names: LATHEO, ORC3, ORC3L
Sequence domains:
Origin recognition complex subunit 4 Chain: D
Molecule details ›
Chain: D
Length: 436 amino acids
Theoretical weight: 50.44 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O43929 (Residues: 1-436; Coverage: 100%)
Gene names: ORC4, ORC4L
Sequence domains:
Origin recognition complex subunit 5 Chain: E
Molecule details ›
Chain: E
Length: 435 amino acids
Theoretical weight: 50.35 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O43913 (Residues: 1-435; Coverage: 100%)
Gene names: ORC5, ORC5L
Sequence domains:
DNA (5'-D(*AP*TP*AP*TP*AP*TP*AP*TP*AP*TP*AP*AP*T)-3') Chain: F
Molecule details ›
Chain: F
Length: 13 nucleotides
Theoretical weight: 3.97 KDa
DNA (5'-D(*AP*TP*TP*AP*TP*AP*TP*AP*TP*AP*TP*AP*T)-3') Chain: G
Molecule details ›
Chain: G
Length: 13 nucleotides
Theoretical weight: 3.96 KDa

Ligands and Environments

3 bound ligands:
Ligand ATP 3 x ATP
Ligand MG 3 x MG
Ligand K 1 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.4Å
Relevant EMDB volumes: EMD-22421
Expression system: Spodoptera frugiperda

Quick links

Citations

4 review citations

Congenital Diseases of DNA Replication: Clinical Phenotypes and Molecular Mechanisms.
Schmit et al. (2021)
3 more