› 7jrm

X-ray diffraction
2Å resolution

The structure of CBM51-2 and INT domains from Clostridium perfringens ZmpB

Released:
DOI: 10.2210/pdb7jrm/pdb
PDB entry 7jrm coloured by chain, front view.
PDB entry 7jrm coloured by chain, side view.
PDB entry 7jrm coloured by chain, top view.
Source organism: Clostridium perfringens ATCC 13124
Primary publication:
Architecturally complex O-glycopeptidases are customized for mucin recognition and hydrolysis.
Pluvinage B, Ficko-Blean E, Noach I, Stuart C, Thompson N, McClure H, Buenbrazo N, Wakarchuk W, Boraston AB
Proc Natl Acad Sci U S A 118 (2021)
PMID: 33658366

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101662 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidase M60 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 462 amino acids
Theoretical weight: 51.2 KDa
Source organism: Clostridium perfringens ATCC 13124
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2YN38 (Residues: 1226-1687; Coverage: 28%)
Gene name: CPF_1489
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: C2
Unit cell:
a: 151.05Å b: 49.16Å c: 78.5Å
α: 90° β: 112.05° γ: 90°
R-values:
R R work R free
0.249 0.248 0.272
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Carbohydrate-active enzymes (CAZymes) in the gut microbiome.
Wardman et al. (2022)
3 more

1 mention without citation

Intravenous Dexmedetomidine Administration Prior Anesthesia Induction With Propofol at 4°C Attenuates Propofol Injection Pain: A Double-Blind, Randomized, Placebo-Controlled Trial.
Lu et al. (2021)