› 7jv5

Electron Microscopy
3Å resolution

Cryo-EM structure of SKF-81297-bound dopamine receptor 1 in complex with Gs protein

Released:
DOI: 10.2210/pdb7jv5/pdb
PDB entry 7jv5 coloured by chain, front view.
PDB entry 7jv5 coloured by chain, side view.
PDB entry 7jv5 coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into the human D1 and D2 dopamine receptor signaling complexes.
Zhuang Y, Xu P, Mao C, Wang L, Krumm B, Zhou XE, Huang S, Liu H, Cheng X, Huang XP, Shen DD, Xu T, Liu YF, Wang Y, Guo J, Jiang Y, Jiang H, Melcher K, Roth BL, Zhang Y, Zhang C, Xu HE
Cell 184 931-942.e18 (2021)
PMID: 33571431
Related structures: EMD-22493

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-210829 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
D(1A) dopamine receptor Chain: R
Molecule details ›
Chain: R
Length: 502 amino acids
Theoretical weight: 55.67 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P21728 (Residues: 1-446; Coverage: 100%)
Gene name: DRD1
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 393 amino acids
Theoretical weight: 45.62 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63092 (Residues: 2-394; Coverage: 100%)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 354 amino acids
Theoretical weight: 39.08 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 67 amino acids
Theoretical weight: 7.43 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 2-68; Coverage: 94%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody 35 Chain: N
Molecule details ›
Chain: N
Length: 135 amino acids
Theoretical weight: 14.85 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

3 bound ligands:
Ligand SK0 1 x SK0
Ligand CLR 6 x CLR
Ligand PLM 6 x PLM
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-22493
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

17 review citations

History of the dopamine hypothesis of antipsychotic action.
Seeman MV. (2021)
16 more

6 mentions without citation

G protein-coupled receptors in neurodegenerative diseases and psychiatric disorders.
Wong et al. (2023)
5 more