› 7k4o

X-ray diffraction
1.65Å resolution

Tannin acyl hydrolase from Aspergillus niger

Released:
DOI: 10.2210/pdb7k4o/pdb
PDB entry 7k4o coloured by chain, front view.
PDB entry 7k4o coloured by chain, side view.
PDB entry 7k4o coloured by chain, top view.
Source organism: Aspergillus niger
Primary publication:
Crystal structure of fungal tannase from Aspergillus niger.
Dong L, McKinstry WJ, Pan L, Newman J, Ren B
Acta Crystallogr D Struct Biol 77 267-277 (2021)
PMID: 33559614

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106927 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Carboxylic ester hydrolase Chain: A
Molecule details ›
Chain: A
Length: 554 amino acids
Theoretical weight: 60.81 KDa
Source organism: Aspergillus niger
Expression system: Aspergillus niger
UniProt:
  • Canonical: A2QIR3 (Residues: 21-574; Coverage: 100%)
Gene name: An04g04430
Sequence domains: Tannase and feruloyl esterase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
7 bound ligands:
Ligand NAG 2 x NAG
Ligand GDE 1 x GDE
Ligand CA 1 x CA
Ligand ZN 4 x ZN
Ligand CL 2 x CL
Ligand NA 1 x NA
Ligand FWN 1 x FWN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P6522
Unit cell:
a: 170.92Å b: 170.92Å c: 111.061Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.153 0.152 0.174
Expression system: Aspergillus niger

Quick links

Citations

1 citations in other articles

Three new yeast species from flowers of Camellia sinensis var. assamica collected in Northern Thailand and their tannin tolerance characterization.
Kanpiengjai et al. (2023)