› 7k66

X-ray diffraction
3.92Å resolution

Structure of Blood Coagulation Factor VIII in Complex with an Anti-C1 Domain Pathogenic Antibody Inhibitor

Released:
DOI: 10.2210/pdb7k66/pdb
PDB entry 7k66 coloured by chain, front view.
PDB entry 7k66 coloured by chain, side view.
PDB entry 7k66 coloured by chain, top view.
Source organisms:
Primary publication:
Structure of blood coagulation factor VIII in complex with an anti-C1 domain pathogenic antibody inhibitor.
Gish JS, Jarvis L, Childers KC, Peters SC, Garrels CS, Smith IW, Spencer HT, Doering CB, Lollar P, Spiegel PC
Blood 137 2981-2986 (2021)
PMID: 33529335

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208205 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
Coagulation factor VIII Chain: A
Molecule details ›
Chain: A
Length: 1467 amino acids
Theoretical weight: 168.29 KDa
Source organisms: Expression system: Cricetulus griseus
UniProt:
  • Canonical: P00451 (Residues: 1-2, 406-761, 2039-2351; Coverage: 28%)
  • Canonical: P12263 (Residues: 3-405, 1438-1820; Coverage: 36%)
Gene names: CF8, F8, F8C
Sequence domains:
2A9 heavy chain Chain: B
Molecule details ›
Chain: B
Length: 223 amino acids
Theoretical weight: 23.98 KDa
Source organism: Mus musculus
Expression system: Mus musculus
2A9 light chain Chain: C
Molecule details ›
Chain: C
Length: 213 amino acids
Theoretical weight: 23.3 KDa
Source organism: Mus musculus
Expression system: Mus musculus

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
4 bound ligands:
Ligand NAG 1 x NAG
Ligand CU 1 x CU
Ligand CA 1 x CA
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P41212
Unit cell:
a: 116.721Å b: 116.721Å c: 371.057Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.226 0.322
Expression systems:
  • Cricetulus griseus
  • Mus musculus

Quick links

Citations

2 review citations

Cryo-EM structures of coagulation factors.
Di Cera et al. (2022)
1 more

2 mentions without citation

Structure of blood coagulation factor VIII in complex with an anti-C1 domain pathogenic antibody inhibitor.
Gish et al. (2021)
1 more