› 7kh9

X-ray diffraction
2.29Å resolution

Crystal structure of OXA-48 K73A in complex with imipenem

Released:
DOI: 10.2210/pdb7kh9/pdb
Model geometry
Fit model/data
PDB entry 7kh9 coloured by chain, front view.
PDB entry 7kh9 coloured by chain, side view.
PDB entry 7kh9 coloured by chain, top view.
Source organism: Klebsiella pneumoniae
Primary publication:
Mechanistic Basis of OXA-48-like β-Lactamases' Hydrolysis of Carbapenems.
Stojanoski V, Hu L, Sankaran B, Wang F, Tao P, Prasad BVV, Palzkill T
ACS Infect Dis 7 445-460 (2021)
PMID: 33492952

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180526 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase OXA-48 Chains: A, B
Molecule details ›
Chains: A, B
Length: 244 amino acids
Theoretical weight: 28.19 KDa
Source organism: Klebsiella pneumoniae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6XEC0 (Residues: 25-265; Coverage: 99%)
Gene names: KPE71T_00045, OXA-48, SAMEA3727706_05517, bla OXA-48, blaOXA-48, bla_4
Sequence domains: Penicillin binding protein transpeptidase domain

Ligands and Environments

2 bound ligands:
Ligand IM2 2 x IM2
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU FR-E SUPERBRIGHT
Unit cell:
a: 44.69Å b: 104.75Å c: 125.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.227
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 citation in other articles

Evolution of β-lactamase-mediated cefiderocol resistance.
Fröhlich et al. (2022)
11 more

3 mentions without citation

Antimicrobial Resistance Conferred by OXA-48 β-Lactamases: Towards a Detailed Mechanistic Understanding.
Hirvonen et al. (2021)
2 more