Function and Biology Details
Reaction catalysed:
(1a) a [histone H3]-N(6),N(6),N(6)-trimethyl-L-lysine(4) + 2-oxoglutarate + O(2) = a [histone H3]-N(6),N(6)-dimethyl-L-lysine(4) + succinate + formaldehyde + CO(2)
Biochemical function:
- not assigned
Biological process:
- not assigned
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
Lysine-specific demethylase 5A and Histone H3.1 (preferred)
PDBe Complex ID:
PDB-CPX-151437 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Lysine-specific demethylase 5A
Molecule details ›
Chain: A
Length: 59 amino acids
Theoretical weight: 6.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: PHD-finger
Length: 59 amino acids
Theoretical weight: 6.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P29375 (Residues: 287-344; Coverage: 3%)
Sequence domains: PHD-finger
Histone H3.1
Molecule details ›
Chain: B
Length: 10 amino acids
Theoretical weight: 1.15 KDa
Source organism: Homo sapiens
Expression system: synthetic construct
UniProt:
Length: 10 amino acids
Theoretical weight: 1.15 KDa
Source organism: Homo sapiens
Expression system: synthetic construct
UniProt:
- Canonical: P68431 (Residues: 2-11; Coverage: 7%)
Ligands and Environments
1 bound ligand:
No modified residues
Experiments and Validation Details
wwPDB Validation report is not available for this NMR entry.
Chemical shift assignment:
77%
Refinement method:
simulated annealing
Expression systems:
- Escherichia coli BL21(DE3)
- synthetic construct
