› 7kpi

X-ray diffraction
1.7Å resolution

Crystal structure of the SPOP MATH domain

Released:
DOI: 10.2210/pdb7kpi/pdb
PDB entry 7kpi coloured by chain, front view.
PDB entry 7kpi coloured by chain, side view.
PDB entry 7kpi coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Intrinsically disordered substrates dictate SPOP subnuclear localization and ubiquitination activity.
Usher ET, Sabri N, Rohac R, Boal AK, Mittag T, Showalter SA
J Biol Chem 296 100693
PMID: 33894201

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112806 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Speckle type BTB/POZ protein Chain: A
Molecule details ›
Chain: A
Length: 142 amino acids
Theoretical weight: 16.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: D6RDG8 (Residues: 28-166; Coverage: 58%)
Gene name: SPOP
Sequence domains: MATH domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: C2
Unit cell:
a: 62.642Å b: 53.246Å c: 41.596Å
α: 90° β: 101.386° γ: 90°
R-values:
R R work R free
0.161 0.159 0.196
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Post-translational modifications in liquid-liquid phase separation: a comprehensive review.
Li et al. (2022)
4 more

2 mentions without citation

Intrinsically disordered substrates dictate SPOP subnuclear localization and ubiquitination activity.
Usher et al. (2021)
1 more