› 7kpl

X-ray diffraction
2.7Å resolution

Crystal structure of hEphB1 in apo form

Released:
DOI: 10.2210/pdb7kpl/pdb
PDB entry 7kpl coloured by chain, front view.
PDB entry 7kpl coloured by chain, side view.
PDB entry 7kpl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Identification of tetracycline combinations as EphB1 tyrosine kinase inhibitors for treatment of neuropathic pain.
Ahmed MS, Wang P, Nguyen NUN, Nakada Y, Menendez-Montes I, Ismail M, Bachoo R, Henkemeyer M, Sadek HA, Kandil ES
Proc Natl Acad Sci U S A 118 (2021)
PMID: 33627480

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157092 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ephrin type-B receptor 1 Chain: A
Molecule details ›
Chain: A
Length: 279 amino acids
Theoretical weight: 31.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P54762 (Residues: 611-889; Coverage: 29%)
Gene names: ELK, EPHB1, EPHT2, HEK6, NET
Sequence domains: Protein tyrosine and serine/threonine kinase

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PTR 1 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 33.057Å b: 91.461Å c: 97.436Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.25
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Drug Repurposing to Target Neuroinflammation and Sensory Neuron-Dependent Pain.
Sisignano et al. (2022)
1 more

1 mention without citation

Identification of tetracycline combinations as EphB1 tyrosine kinase inhibitors for treatment of neuropathic pain.
Ahmed et al. (2021)