› 7kuf

X-ray diffraction
2.6Å resolution

Transcription activation subcomplex with WhiB7 bound to SigmaAr4-RNAP Beta flap tip chimera and DNA

Released:
DOI: 10.2210/pdb7kuf/pdb
PDB entry 7kuf coloured by chain, front view.
PDB entry 7kuf coloured by chain, side view.
PDB entry 7kuf coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into the functional divergence of WhiB-like proteins in Mycobacterium tuberculosis.
Wan T, Horová M, Beltran DG, Li S, Wong HX, Zhang LM
Mol Cell 81 2887-2900.e5 (2021)
PMID: 34171298

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-121508 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Probable transcriptional regulator WhiB7 Chain: A
Molecule details ›
Chain: A
Length: 92 amino acids
Theoretical weight: 10.15 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6MX01 (Residues: 1-92; Coverage: 100%)
Gene names: Rv3197.1, Rv3197A, whiB7
Sequence domains:
DNA-directed RNA polymerase subunit beta; RNA polymerase sigma factor SigA Chain: B
Molecule details ›
Chain: B
Length: 112 amino acids
Theoretical weight: 12.65 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WGI1 (Residues: 446-528; Coverage: 16%)
  • Canonical: P9WGY9 (Residues: 815-829; Coverage: 1%)
Gene names: MTCI376.08c, MTCY05A6.24, Rv0667, Rv2703, mysA, rpoB, rpoD, rpoV, sigA
Sequence domains: Sigma-70, region 4
DNA (5'-D(*CP*AP*GP*AP*AP*AP*AP*TP*CP*GP*GP*TP*TP*GP*TP*GP*GP*T)-3') Chain: C
Molecule details ›
Chain: C
Length: 18 nucleotides
Theoretical weight: 5.6 KDa
DNA (5'-D(*GP*AP*CP*CP*AP*CP*AP*AP*CP*CP*GP*AP*TP*TP*TP*TP*CP*T)-3') Chain: D
Molecule details ›
Chain: D
Length: 18 nucleotides
Theoretical weight: 5.44 KDa

Ligands and Environments

1 bound ligand:
Ligand SF4 1 x SF4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P3221
Unit cell:
a: 69.254Å b: 69.254Å c: 167.374Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.221 0.255
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Metalloprotein enabled redox signal transduction in microbes.
Williams et al. (2023)
5 other articles

3 mentions without citation

Structural insights into the functional divergence of WhiB-like proteins in Mycobacterium tuberculosis.
Wan et al. (2021)
2 more