› 7kwo

Electron Microscopy
2.9Å resolution

rFVIIIFc-VWF-XTEN (BIVV001)

Released:
DOI: 10.2210/pdb7kwo/pdb
PDB entry 7kwo coloured by chain, front view.
PDB entry 7kwo coloured by chain, side view.
PDB entry 7kwo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular determinants of the factor VIII/von Willebrand factor complex revealed by BIVV001 cryo-electron microscopy.
Fuller JR, Knockenhauer KE, Leksa NC, Peters RT, Batchelor JD
Blood 137 2970-2980 (2021)
PMID: 33569592
Related structures: EMD-23057

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132520 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Coagulation factor VIII Chain: A
Molecule details ›
Chain: A
Length: 1965 amino acids
Theoretical weight: 218.87 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P00451 (Residues: 1-2351; Coverage: 74%)
Gene names: F8, F8C
Sequence domains:
Immunoglobulin heavy constant gamma 1; von Willebrand factor Chain: V
Molecule details ›
Chain: V
Length: 1646 amino acids
Theoretical weight: 179.05 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P04275 (Residues: 1-1241, 1242-1253, 1254-1256; Coverage: 44%)
  • Canonical: P01857 (Residues: 12-97, 98-102, 103-328; Coverage: 77%)
Gene names: F8VWF, IGHG1, VWF
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
4 bound ligands:
Ligand NAG 3 x NAG
Ligand CA 2 x CA
Ligand ZN 1 x ZN
Ligand CU 1 x CU
1 modified residue:
Ligand TYS 1 x TYS

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-23057
Expression system: Homo sapiens

Quick links

Citations

3 review citations

Efanesoctocog Alfa: First Approval.
Keam SJ. (2023)
2 more

4 mentions without citation

Molecular determinants of the factor VIII/von Willebrand factor complex revealed by BIVV001 cryo-electron microscopy.
Fuller et al. (2021)
3 more