› 7kyp

X-ray diffraction
2.9Å resolution

PsaBC from Streptococcus pneumoniae in complex with Fab

Released:
DOI: 10.2210/pdb7kyp/pdb
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PDB entry 7kyp coloured by chain, front view.
PDB entry 7kyp coloured by chain, side view.
PDB entry 7kyp coloured by chain, top view.
Source organism: Streptococcus pneumoniae D39
Primary publication:
The structural basis of bacterial manganese import.
Neville SL, Sjöhamn J, Watts JA, MacDermott-Opeskin H, Fairweather SJ, Ganio K, Carey Hulyer A, McGrath AP, Hayes AJ, Malcolm TR, Davies MR, Nomura N, Iwata S, O'Mara ML, Maher MJ, McDevitt CA
OpenAccess logo Sci Adv 7 (2021)
PMID: 34362732

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101721 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ABC transporter domain-containing protein Chains: A, C, E, G, I, K, M, O
Molecule details ›
Chains: A, C, E, G, I, K, M, O
Length: 240 amino acids
Theoretical weight: 26.88 KDa
Source organism: Streptococcus pneumoniae D39
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H2ZNF3 (Residues: 1-240; Coverage: 100%)
Gene names: SPD_1461, psaB
Sequence domains: ABC transporter
Manganese import system permease protein ScaB Chains: B, D, F, H, J, L, N, P
Molecule details ›
Chains: B, D, F, H, J, L, N, P
Length: 290 amino acids
Theoretical weight: 31.08 KDa
Source organism: Streptococcus pneumoniae D39
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H2ZPI2 (Residues: 1-282; Coverage: 100%)
Gene names: SPD_1462, psaC
Sequence domains: ABC 3 transport family

Ligands and Environments

2 bound ligands:
Ligand PO4 8 x PO4
Ligand MA4 4 x MA4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P1
Unit cell:
a: 92.55Å b: 129.56Å c: 169.62Å
α: 95.35° β: 92.21° γ: 98.66°
R-values:
R R work R free
0.261 0.26 0.281
Expression system: Escherichia coli

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Citations

3 review citations

Managing Manganese: The Role of Manganese Homeostasis in Streptococcal Pathogenesis.
Aggarwal et al. (2022)
2 more

1 mention without citation

Titratable transmembrane residues and a hydrophobic plug are essential for manganese import via the Bacillus anthracis ABC transporter MntBC-A.
Kuznetsova et al. (2021)

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