› 7l0a

X-ray diffraction
1.6Å resolution

Crystal structure of s-formylglutathione hydrolase (FrmB) from Staphylococcus aureus, apoenzyme

Released:
DOI: 10.2210/pdb7l0a/pdb
PDB entry 7l0a coloured by chain, front view.
PDB entry 7l0a coloured by chain, side view.
PDB entry 7l0a coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Structure-guided microbial targeting of antistaphylococcal prodrugs.
Miller JJ, Shah IT, Hatten J, Barekatain Y, Mueller EA, Moustafa AM, Edwards RL, Dowd CS, Hoops GC, Johnson RJ, Planet PJ, Muller FL, Jez JM, Odom John AR
Elife 10 (2021)
PMID: 34279224

Function and Biology Details

Reaction catalysed: 
S-formylglutathione + H(2)O = glutathione + formate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173411 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-formylglutathione hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 261 amino acids
Theoretical weight: 29.93 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q2FUY3 (Residues: 1-253; Coverage: 100%)
Gene name: SAOUHSC_02962
Sequence domains: Putative esterase

Ligands and Environments

1 bound ligand:
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 128.303Å b: 80.5Å c: 66.651Å
α: 90° β: 113.84° γ: 90°
R-values:
R R work R free
0.158 0.157 0.177
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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Citations

1 review citation

An Update Review of Approaches to Multiple Action-Based Antibacterials.
Bremner JB. (2023)
1 other article

2 mentions without citation

Newly discovered Staphylococcus aureus serine hydrolase probe and drug targets.
Fellner M. (2022)
1 more