› 7l1t

X-ray diffraction
2.25Å resolution

Crystal structure of human holo SepSecS

Released:
DOI: 10.2210/pdb7l1t/pdb
PDB entry 7l1t coloured by chain, front view.
PDB entry 7l1t coloured by chain, side view.
PDB entry 7l1t coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the tRNA-dependent activation of the terminal complex of selenocysteine synthesis in humans.
Puppala AK, Castillo Suchkou J, French RL, Kiernan KA, Simonović M
Nucleic Acids Res (2023)
PMID: 36929010

Function and Biology Details

Reaction catalysed: 
O-phospho-L-seryl-tRNA(Sec) + selenophosphate + H(2)O = L-selenocysteinyl-tRNA(Sec) + 2 phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190801 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
O-phosphoseryl-tRNA(Sec) selenium transferase Chain: A
Molecule details ›
Chain: A
Length: 521 amino acids
Theoretical weight: 57.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HD40 (Residues: 1-501; Coverage: 100%)
Gene names: SEPSECS, TRNP48
Sequence domains: O-phosphoseryl-tRNA(Sec) selenium transferase, SepSecS

Ligands and Environments


Cofactor: Ligand PLR 1 x PLR
1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P622
Unit cell:
a: 154.216Å b: 154.216Å c: 76.257Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.192 0.23
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

Structural basis for the tRNA-dependent activation of the terminal complex of selenocysteine synthesis in humans.
Puppala et al. (2023)