› 7l1y

X-ray diffraction
1.2Å resolution

Unlocking the structural features for the exo-xylobiosidase activity of an unusual GH11 member identified in a compost-derived consortium-xylobiose complex

Released:
DOI: 10.2210/pdb7l1y/pdb
PDB entry 7l1y coloured by chain, front view.
PDB entry 7l1y coloured by chain, side view.
PDB entry 7l1y coloured by chain, top view.
Source organism: unidentified
Primary publication:
Unlocking the structural features for the xylobiohydrolase activity of an unusual GH11 member identified in a compost-derived consortium.
Kadowaki MAS, Briganti L, Evangelista DE, Echevarría-Poza A, Tryfona T, Pellegrini VOA, Nakayama DG, Dupree P, Polikarpov I
Biotechnol Bioeng 118 4052-4064 (2021)
PMID: 34232504

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104238 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
endo-1,4-beta-xylanase Chain: A
Molecule details ›
Chain: A
Length: 247 amino acids
Theoretical weight: 27.68 KDa
Source organism: unidentified
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A2H4TGG5 (Residues: 22-268; Coverage: 100%)
Gene name: xyn11a
Sequence domains: Glycosyl hydrolases family 11

Ligands and Environments

Carbohydrate polymer : NEW Components: XYP
Carbohydrate polymer
1 bound ligand:
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P43212
Unit cell:
a: 55.958Å b: 55.958Å c: 163.276Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.18 0.203
Expression system: Escherichia coli BL21(DE3)

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