› 7l3y

X-ray diffraction
1.18Å resolution

Crystal structure of oxy-I107E CuB myoglobin (I107E L29H F43H sperm whale myoglobin; partial occupancy)

Released:
DOI: 10.2210/pdb7l3y/pdb
Model geometry
Fit model/data
PDB entry 7l3y coloured by chain, front view.
PDB entry 7l3y coloured by chain, side view.
PDB entry 7l3y coloured by chain, top view.
Source organism: Physeter catodon
Primary publication:
An Engineered Glutamate in Biosynthetic Models of Heme-Copper Oxidases Drives Complete Product Selectivity by Tuning the Hydrogen-Bonding Network.
Petrik ID, Davydov R, Kahle M, Sandoval B, Dwaraknath S, Ädelroth P, Hoffman B, Lu Y
Biochemistry 60 346-355 (2021)
PMID: 33464878

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135409 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myoglobin Chain: A
Molecule details ›
Chain: A
Length: 154 amino acids
Theoretical weight: 17.4 KDa
Source organism: Physeter catodon
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02185 (Residues: 1-154; Coverage: 100%)
Gene name: MB
Sequence domains: Globin

Ligands and Environments

3 bound ligands:
Ligand HEM 1 x HEM
Ligand PER 1 x PER
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P212121
Unit cell:
a: 39.845Å b: 46.982Å c: 78.034Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.157 0.19
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Rejigging Electron and Proton Transfer to Transition between Dioxygenase, Monooxygenase, Peroxygenase, and Oxygen Reduction Activity: Insights from Bioinspired Constructs of Heme Enzymes.
Mukherjee et al. (2021)
1 other article

3 mentions without citation

Designing Artificial Metalloenzymes by Tuning of the Environment beyond the Primary Coordination Sphere.
Van Stappen et al. (2022)
2 more