› 7l5r

X-ray diffraction
1.65Å resolution

Crystal Structure of the Oxacillin-hydrolyzing Class D Extended-spectrum Beta-lactamase OXA-14 from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb7l5r/pdb
Model geometry
Fit model/data
PDB entry 7l5r coloured by chain, front view.
PDB entry 7l5r coloured by chain, side view.
PDB entry 7l5r coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Functional and Structural Characterization of OXA-935, a Novel OXA-10-Family β-Lactamase from Pseudomonas aeruginosa.
Pincus NB, Rosas-Lemus M, Gatesy SWM, Bertucci HK, Brunzelle JS, Minasov G, Shuvalova LA, Lebrun-Corbin M, Satchell KJF, Ozer EA, Hauser AR, Bachta KER
OpenAccess logo Antimicrob Agents Chemother 66 e0098522 (2022)
PMID: 36129295

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146959 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Beta-lactamase OXA-10 Chain: A
Molecule details ›
Chain: A
Length: 248 amino acids
Theoretical weight: 27.83 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14489 (Residues: 21-266; Coverage: 100%)
Gene names: OXA-10, bla, oxa10, pse2
Sequence domains: Penicillin binding protein transpeptidase domain
Beta-lactamase OXA-10 Chain: B
Molecule details ›
Chain: B
Length: 248 amino acids
Theoretical weight: 27.78 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14489 (Residues: 21-266; Coverage: 100%)
Gene names: OXA-10, bla, oxa10, pse2
Sequence domains: Penicillin binding protein transpeptidase domain

Ligands and Environments

2 bound ligands:
Ligand GOL 3 x GOL
Ligand SO4 22 x SO4
1 modified residue:
Ligand KCX 1 x KCX

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 48.813Å b: 96.346Å c: 125.137Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.164 0.19
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Prioritization of Critical Factors for Surveillance of the Dissemination of Antibiotic Resistance in Pseudomonas aeruginosa: A Systematic Review.
Lee et al. (2023)

1 mention without citation

Functional and Structural Characterization of OXA-935, a Novel OXA-10-Family β-Lactamase from Pseudomonas aeruginosa.
Pincus et al. (2022)