› 7l6n

Electron Microscopy
7Å resolution

The Mycobacterium tuberculosis ClpB disaggregase hexamer structure with three locally refined ClpB middle domains and three DnaK nucleotide binding domains

Released:
DOI: 10.2210/pdb7l6n/pdb
PDB entry 7l6n coloured by chain, front view.
PDB entry 7l6n coloured by chain, side view.
PDB entry 7l6n coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Structural basis for aggregate dissolution and refolding by the Mycobacterium tuberculosis ClpB-DnaK bi-chaperone system.
Yin Y, Feng X, Yu H, Fay A, Kovach A, Glickman MS, Li H
Cell Rep 35 109166 (2021)
PMID: 34038719
Related structures: EMD-23206

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-161800 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Chaperone protein ClpB Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 848 amino acids
Theoretical weight: 92.69 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WPD1 (Residues: 1-848; Coverage: 100%)
Gene names: MTV036.19c, Rv0384c, clpB
Sequence domains:
Substrate Chain: N
Molecule details ›
Chain: N
Length: 33 amino acids
Theoretical weight: 2.83 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
Chaperone protein DnaK Chains: I, J, K
Molecule details ›
Chains: I, J, K
Length: 625 amino acids
Theoretical weight: 66.91 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WMJ9 (Residues: 1-625; Coverage: 100%)
Gene names: MTCY13E10.10, Rv0350, dnaK
Sequence domains: Hsp70 protein

Ligands and Environments

2 bound ligands:
Ligand AGS 10 x AGS
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 7Å
Relevant EMDB volumes: EMD-23206
Expression system: Escherichia coli

Quick links

Citations

1 review citation

AAA+ proteins: one motor, multiple ways to work.
Lin et al. (2022)
4 other articles

2 mentions without citation

Electron microscopy holdings of the Protein Data Bank: the impact of the resolution revolution, new validation tools, and implications for the future.
Burley et al. (2022)
1 more