› 7lao

X-ray diffraction
1.92Å resolution

Crystal structure of aminoglycoside acetyltransferase AAC(3)-IIb

Released:
DOI: 10.2210/pdb7lao/pdb
PDB entry 7lao coloured by chain, front view.
PDB entry 7lao coloured by chain, side view.
PDB entry 7lao coloured by chain, top view.
Source organism: Serratia marcescens
Primary publication:
Structural and molecular rationale for the diversification of resistance mediated by the Antibiotic_NAT family.
Stogios PJ, Bordeleau E, Xu Z, Skarina T, Evdokimova E, Chou S, Diorio-Toth L, D'Souza AW, Patel S, Dantas G, Wright GD, Savchenko A
Commun Biol 5 263 (2022)
PMID: 35338238

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + a 2-deoxystreptamine antibiotic = CoA + N(3)-acetyl-2-deoxystreptamine antibiotic
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169579 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminoglycoside N(3)-acetyltransferase III Chain: A
Molecule details ›
Chain: A
Length: 271 amino acids
Theoretical weight: 29.02 KDa
Source organism: Serratia marcescens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q01515 (Residues: 1-269; Coverage: 100%)
Gene name: aac3-Vb
Sequence domains: Aminoglycoside 3-N-acetyltransferase

Ligands and Environments

2 bound ligands:
Ligand EPE 1 x EPE
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 43.174Å b: 61.434Å c: 112.031Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.229
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Restoring susceptibility to aminoglycosides: identifying small molecule inhibitors of enzymatic inactivation.
Magaña et al. (2023)
1 other article

1 mention without citation

Structural and molecular rationale for the diversification of resistance mediated by the Antibiotic_NAT family.
Stogios et al. (2022)