› 7lbe

Electron Microscopy
2.9Å resolution

CryoEM structure of the HCMV Trimer gHgLgO in complex with neutralizing fabs 13H11 and MSL-109

Released:
DOI: 10.2210/pdb7lbe/pdb
PDB entry 7lbe coloured by chain, front view.
PDB entry 7lbe coloured by chain, side view.
PDB entry 7lbe coloured by chain, top view.
Source organisms:
Primary publication:
Structures of HCMV Trimer reveal the basis for receptor recognition and cell entry.
Kschonsak M, Rougé L, Arthur CP, Hoangdung H, Patel N, Kim I, Johnson MC, Kraft E, Rohou AL, Gill A, Martinez-Martin N, Payandeh J, Ciferri C
Cell 184 1232-1244.e16 (2021)
PMID: 33626330
Related structures: EMD-23252

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
PDBe Complex ID:
PDB-CPX-207852 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (9 distinct):
Envelope glycoprotein H Chain: A
Molecule details ›
Chain: A
Length: 767 amino acids
Theoretical weight: 87.31 KDa
Source organism: Human herpesvirus 5 strain Merlin
Expression system: Homo sapiens
UniProt:
  • Canonical: Q6SW67 (Residues: 1-715; Coverage: 96%)
Gene names: UL75, gH
Sequence domains:
Envelope glycoprotein L Chain: B
Molecule details ›
Chain: B
Length: 278 amino acids
Theoretical weight: 30.85 KDa
Source organism: Human herpesvirus 5 strain Merlin
Expression system: Homo sapiens
UniProt:
  • Canonical: F5HCH8 (Residues: 1-278; Coverage: 100%)
Gene names: UL115, gL
Sequence domains: Cytomegalovirus glycoprotein L
Envelope glycoprotein O Chain: C
Fab 13H11 light chain Chain: E
Fab 13H11 heavy chain Chain: F
Fab MSL-109 light chain Chain: G
Fab MSL-109 heavy chain Chain: H

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, MAN, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand NAG 16 x NAG
Ligand MAN 1 x MAN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 2.9Å
Relevant EMDB volumes: EMD-23252
Expression systems:
  • Homo sapiens
  • Escherichia coli

Quick links

Citations

5 review citations

Deep Mutational Scanning of Viral Glycoproteins and Their Host Receptors.
Narayanan et al. (2021)
4 more

3 mentions without citation

Antibodies to combat viral infections: development strategies and progress.
Pantaleo et al. (2022)
2 more